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Average recovery/separation of white blood cells (WBCs) or peripheral blood mononuclear cells (PBMC) from whole blood with three commonly-used methods. Error bars are one standard deviation of measurements from five samples. WBC filters efficiently capture more than 90% WBCs from whole blood.

Journal: Sensors & Diagnostics

Article Title: Lateral flow assay-based detection of nuclear fusion oncoprotein: implications for screening of acute promyelocytic leukemia †

doi: 10.1039/d4sd00357h

Figure Lengend Snippet: Average recovery/separation of white blood cells (WBCs) or peripheral blood mononuclear cells (PBMC) from whole blood with three commonly-used methods. Error bars are one standard deviation of measurements from five samples. WBC filters efficiently capture more than 90% WBCs from whole blood.

Article Snippet: Cryopreserved human peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells, LLC.

Techniques: Standard Deviation

Sensitive APL-LFA-based detection of PML–RARα fusion protein in NB4 cell lysate spiked into PBMC lysate. NB4 cell lysate was mixed with PBMC lysate in various percentages (in a total of 33 000 cells). The LFA signals were measured using a portable LFA reader and quantified using ImageJ. Error bars are one standard deviation of three measurements. The cutoff (dashed line) is the average plus 1.645 times the standard deviation of the negative tests (100% healthy PBMC lysate) plus 1.645 times the standard deviation of the low positive tests (5% NB4 cell lysate) giving a limit of detection (LoD) of 11% NB4 cell lysate in a total of 33 000 cells.

Journal: Sensors & Diagnostics

Article Title: Lateral flow assay-based detection of nuclear fusion oncoprotein: implications for screening of acute promyelocytic leukemia †

doi: 10.1039/d4sd00357h

Figure Lengend Snippet: Sensitive APL-LFA-based detection of PML–RARα fusion protein in NB4 cell lysate spiked into PBMC lysate. NB4 cell lysate was mixed with PBMC lysate in various percentages (in a total of 33 000 cells). The LFA signals were measured using a portable LFA reader and quantified using ImageJ. Error bars are one standard deviation of three measurements. The cutoff (dashed line) is the average plus 1.645 times the standard deviation of the negative tests (100% healthy PBMC lysate) plus 1.645 times the standard deviation of the low positive tests (5% NB4 cell lysate) giving a limit of detection (LoD) of 11% NB4 cell lysate in a total of 33 000 cells.

Article Snippet: Cryopreserved human peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells, LLC.

Techniques: Standard Deviation